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A more general method called linked (associated, programmed) searching allows advanced searching of primer-template binding sites in a variety of scenarios, including that of in silico PCR. Early Christian inscriptions of Munster The Iron Grail (Merlin Codex) Thinking Mathematically 6Th Edition By Blitzer Mp4 Sequence Manipulation Suite: PCR Primer Stats: PCR Primer Stats accepts a list of PCR primer sequences and returns a report describing the properties of each primer, including melting temperature, percent GC content, and PCR suitability. Adjustments of the components in PCR is commonly used for optimal performance. The divalent magnesium ion (Mg ++) is required for PCR polymerase activity.Lower concentrations Mg ++ will increase replication fidelity, while higher concentrations will introduce more mutations. [citation needed]Denaturants(such as DMSO) can increase amplification specificity by destabilizing non-specific primer ... 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