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Catalytic Bioscavengers Against Toxic Esters, an Alternative Approach for Prophylaxis and Treatments of Poisonings Gibson assembly® is a molecular cloning method which allows for the joining of multiple DNA fragments in a single, isothermal reaction. It was created by Daniel G. Gibson, Vice President of DNA Technology at Synthetic Genomics Inc (SGI), in collaboration with the J. Craig Venter Institute (JCVI). The Lord Reigneth Financial Times World Desk Reference 2003 Stihl Sr 200 Repair Manual Sivrisinek Sahili A Biography of James A. Garfield Helicases are often used to separate strands of a DNA double helix or a self-annealed RNA molecule using the energy from ATP hydrolysis, a process characterized by the breaking of hydrogen bonds between annealed nucleotide bases.They also function to remove nucleic acid-associated proteins and catalyze homologous DNA recombination. 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